In a study recently published in Neuron and featured in Nature Methods, the lab of Dr. Botond Roska performed targeted single-cell-initiated transsynaptic tracing to label retinal ganglion cells that provide input to individual principal cells in the mouse lateral geniculate nucleus (LGN).

Reconstruction of different ganglion cells all connected to the same principal cell in LGN. The cells are color-coded according to their morphological cell type.

Subsequently, more than 500 labeled ganglion cells were reconstructed using ariadne-service and PyKNOSSOS. An analysis of the morphology and innervation patterns of the individual ganglion cells revealed distinct modes of ganglion cell input integration by LGN principal cells.